Chapter title |
The Peptide Microarray-Based Assay for Kinase Functionality and Inhibition Study
|
---|---|
Chapter number | 18 |
Book title |
Peptide Microarrays
|
Published in |
Methods in molecular biology (Clifton, N.J.), January 2009
|
DOI | 10.1007/978-1-60327-394-7_18 |
Pubmed ID | |
Book ISBNs |
978-1-60327-393-0, 978-1-60327-394-7
|
Authors |
Zhenxin Wang, Jung-Hao Wang, Liang-Ju Chien, Tsung-Min Hsieh, Ching-Hsing Luo, Wen-Pin Chou, Ping-Hei Chen, Pei-Jer Chen, Da-Sheng Lee, Gwo-Bin Lee |
Abstract |
We report a microarray format for the detection of kinase functionality/inhibition based on marking peptide phosphorylation/biotinylation events by the attachment of gold nanoparticles followed by silver deposition for signal enhancement. The detection principle is resonance light scattering (RLS) or surface-enhanced Raman spectroscopy (SERS). Alpha-catalytic subunit of cyclic adenosine 5'-monophosphate (cAMP) dependent protein kinase (PKA) and its well-known substrate, kemptide, are used for the purpose of monitoring phosphorylation and inhibition. As expected, highly selective inhibition of PKA is demonstrated with the four inhibitors: H89, HA1077, mallotoxin and KN62. Furthermore, inhibition assay with inhibitors demonstrate the ability to detect kinase inhibition as well as derive IC50 (half maximal inhibitory concentration) plots. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 8 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Professor > Associate Professor | 3 | 38% |
Professor | 1 | 13% |
Student > Bachelor | 1 | 13% |
Researcher | 1 | 13% |
Student > Ph. D. Student | 1 | 13% |
Other | 0 | 0% |
Unknown | 1 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 2 | 25% |
Medicine and Dentistry | 2 | 25% |
Computer Science | 1 | 13% |
Chemistry | 1 | 13% |
Materials Science | 1 | 13% |
Other | 0 | 0% |
Unknown | 1 | 13% |