| Chapter title |
Detection of E2F-Induced Transcriptional Activity Using a Dual Luciferase Reporter Assay
|
|---|---|
| Chapter number | 14 |
| Book title |
The Retinoblastoma Protein
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7565-5_14 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7564-8, 978-1-4939-7565-5
|
| Authors |
Ainhoa Iglesias-Ara, Nerea Osinalde, Ana M. Zubiaga |
| Abstract |
The E2F transcription factors are key targets for the retinoblastoma (RB) tumor suppressor function. The active or inactive status of RB determines the degree by which E2F-dependent gene expression will occur in a given condition. Changes in transcriptional activity in response to extracellular or intracellular stimuli are frequently measured using genetic reporter assays. In particular, dual luciferase reporter assays are most recommended for this purpose because of their improved experimental accuracy. Here we illustrate the usefulness of the dual luciferase reporter assay to detect E2F-mediated transcriptional activity upon overexpression of E2F1 in cultured cells as readout for RB status and function. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 15 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 33% |
| Lecturer | 1 | 7% |
| Student > Doctoral Student | 1 | 7% |
| Librarian | 1 | 7% |
| Professor | 1 | 7% |
| Other | 3 | 20% |
| Unknown | 3 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 40% |
| Agricultural and Biological Sciences | 2 | 13% |
| Arts and Humanities | 1 | 7% |
| Immunology and Microbiology | 1 | 7% |
| Social Sciences | 1 | 7% |
| Other | 1 | 7% |
| Unknown | 3 | 20% |