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Mendeley readers
Chapter title |
Generation of Intramolecular FRET Probes via Noncanonical Amino Acid Mutagenesis
|
---|---|
Chapter number | 21 |
Book title |
Noncanonical Amino Acids
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7574-7_21 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7573-0, 978-1-4939-7574-7
|
Authors |
Simone Brand, Yao-Wen Wu |
Abstract |
Förster resonance energy transfer (FRET) probes are powerful tools to monitor protein-protein interactions and enzyme activities in a spatiotemporal manner in live cells. Using a combination of noncanonical amino acid (ncAA) mutagenesis and bioorthogonal labeling, we have developed intramolecular FRET probes consisting of a fluorescent protein and an organic dye within an individual protein. Herein we present a general approach to establish intramolecular FRET probes for imaging of protein activity in live cells. |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Doctoral Student | 1 | 17% |
Student > Ph. D. Student | 1 | 17% |
Student > Master | 1 | 17% |
Researcher | 1 | 17% |
Professor > Associate Professor | 1 | 17% |
Other | 0 | 0% |
Unknown | 1 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 3 | 50% |
Biochemistry, Genetics and Molecular Biology | 1 | 17% |
Nursing and Health Professions | 1 | 17% |
Engineering | 1 | 17% |