Title |
Utilizing Gold Nanoparticle Probes to Visually Detect DNA Methylation
|
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Published in |
Discover Nano, June 2016
|
DOI | 10.1186/s11671-016-1487-5 |
Pubmed ID | |
Authors |
Kui Chen, Mingyi Zhang, Ya-Nan Chang, Lin Xia, Weihong Gu, Yanxia Qin, Juan Li, Suxia Cui, Gengmei Xing |
Abstract |
The surface plasmon resonance (SPR) effect endows gold nanoparticles (GNPs) with the ability to visualize biomolecules. In the present study, we designed and constructed a GNP probe to allow the semi-quantitative analysis of methylated tumor suppressor genes in cultured cells. To construct the probe, the GNP surfaces were coated with single-stranded DNA (ssDNA) by forming Au-S bonds. The ssDNA contains a thiolated 5'-end, a regulatory domain of 12 adenine nucleotides, and a functional domain with absolute pairing with methylated p16 sequence (Met-p16). The probe, paired with Met-p16, clearly changed the color of aggregating GNPs probe in 5 mol/L NaCl solution. Utilizing the probe, p16 gene methylation in HCT116 cells was semi-quantified. Further, the methylation of E-cadherin, p15, and p16 gene in Caco2, HepG2, and HCT116 cell lines were detected by the corresponding probes, constructed with three domains. This simple and cost-effective method was useful for the diagnosis of DNA methylation-related diseases. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 22 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Bachelor | 6 | 27% |
Student > Ph. D. Student | 4 | 18% |
Other | 3 | 14% |
Student > Master | 2 | 9% |
Student > Postgraduate | 2 | 9% |
Other | 2 | 9% |
Unknown | 3 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 5 | 23% |
Engineering | 4 | 18% |
Biochemistry, Genetics and Molecular Biology | 3 | 14% |
Medicine and Dentistry | 3 | 14% |
Physics and Astronomy | 1 | 5% |
Other | 3 | 14% |
Unknown | 3 | 14% |