Chapter title |
Subcellular localization of legionella Dot/Icm effectors.
|
---|---|
Chapter number | 20 |
Book title |
Legionella
|
Published in |
Methods in molecular biology, January 2013
|
DOI | 10.1007/978-1-62703-161-5-20 |
Pubmed ID | |
Book ISBNs |
978-1-62703-160-8, 978-1-62703-161-5
|
Authors |
Vogrin, Adam J, Mousnier, Aurelie, Frankel, Gad, Hartland, Elizabeth L, Adam J. Vogrin, Aurelie Mousnier, Gad Frankel, Elizabeth L. Hartland, Vogrin, Adam J., Hartland, Elizabeth L. |
Abstract |
The translocation of effector proteins by the Dot/Icm type IV secretion system is central to the ability of Legionella pneumophila to persist and replicate within eukaryotic cells. The subcellular localization of translocated Dot/Icm proteins in host cells provides insight into their function. Through co-staining with host cell markers, effector proteins may be localized to specific subcellular compartments and membranes, which frequently reflects their host cell target and mechanism of action. In this chapter, we describe protocols to (1) localize effector proteins within cells by ectopic expression using green fluorescent protein fusions and (2) localize effector proteins within infected cells using epitope-tagged effector proteins and immuno-fluorescence microscopy. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 3 | 50% |
Researcher | 1 | 17% |
Lecturer | 1 | 17% |
Student > Doctoral Student | 1 | 17% |
Readers by discipline | Count | As % |
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Immunology and Microbiology | 2 | 33% |
Agricultural and Biological Sciences | 2 | 33% |
Chemical Engineering | 1 | 17% |
Unknown | 1 | 17% |