Membrane Proteins Production for Structural Analysis

Chapter 1 Membrane Protein Production for Structural Analysis

Chapter 2 Membrane Protein Quality Control in Cell-Free Expression Systems: Tools, Strategies and Case Studies

Chapter 3 Bacterial Expression and Stabilization of GPCRs

Chapter 4 Membrane Protein Production in Escherichia coli: Overview and Protocols

Chapter 5 Lactococcus lactis: Recent Developments in Functional Expression of Membrane Proteins

Chapter 6 Overexpression of Membrane Proteins in Saccharomyces cerevisiae for Structural and Functional Studies: A Focus on the Rabbit Ca 2+ -ATPase Serca1a and on the Yeast Lipid “Flippase” Complex Drs2p/Cdc50p

Chapter 7 Amphipols: A General Introduction and Some Protocols

Chapter 8 New Amphiphiles to Handle Membrane Proteins: “Ménage à Trois” Between Chemistry, Physical Chemistry, and Biochemistry

Chapter 9 Building Model Membranes with Lipids and Proteins: Dangers and Challenges

Chapter 10 Analytical Ultracentrifugation and Size-Exclusion Chromatography Coupled with Light Scattering for the Characterization of Membrane Proteins in Solution

Chapter 11 Lipidic Cubic Phase Technologies for Structural Studies of Membrane Proteins

Chapter 12 Micelles, Bicelles, Amphipols, Nanodiscs, Liposomes, or Intact Cells: The Hitchhiker’s Guide to the Study of Membrane Proteins by NMR

Chapter 13 Foundations of Biomolecular Simulations: A Critical Introduction to Homology Modeling, Molecular Dynamics Simulations, and Free Energy Calculations of Membrane Proteins

Chapter 14 Structural Studies of TSPO, a Mitochondrial Membrane Protein

Chapter 15 Foundations of Biomolecular Simulations: A Critical Introduction to Homology Modeling, Molecular Dynamics Simulations, and Free Energy Calculations of Membrane Proteins