Chapter title |
Identification of Essential Genes and Synthetic Lethal Gene Combinations in Escherichia coli K-12.
|
---|---|
Chapter number | 4 |
Book title |
Gene Essentiality
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2398-4_4 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2397-7, 978-1-4939-2398-4
|
Authors |
Hirotada Mori, Tomoya Baba, Katsushi Yokoyama, Rikiya Takeuchi, Wataru Nomura, Kazuichi Makishi, Yuta Otsuka, Hitomi Dose, Barry L Wanner, Barry L. Wanner, Mori, Hirotada, Baba, Tomoya, Yokoyama, Katsushi, Takeuchi, Rikiya, Nomura, Wataru, Makishi, Kazuichi, Otsuka, Yuta, Dose, Hitomi, Wanner, Barry L. |
Abstract |
Here we describe the systematic identification of single genes and gene pairs, whose knockout causes lethality in Escherichia coli K-12. During construction of precise single-gene knockout library of E. coli K-12, we identified 328 essential gene candidates for growth in complex (LB) medium. Upon establishment of the Keio single-gene deletion library, we undertook the development of the ASKA single-gene deletion library carrying a different antibiotic resistance. In addition, we developed tools for identification of synthetic lethal gene combinations by systematic construction of double-gene knockout mutants. We introduce these methods herein. |
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Mendeley readers
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Researcher | 2 | 4% |
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Mathematics | 1 | 2% |
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