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Mendeley readers
Chapter title |
Quantitative Analysis of Central Energy Metabolism in Cell Culture Samples
|
---|---|
Chapter number | 25 |
Book title |
Clinical Metabolomics
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-7592-1_25 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7591-4, 978-1-4939-7592-1
|
Authors |
Sarantos Kostidis |
Abstract |
Nuclear magnetic resonance (NMR) is one of the key analytical platforms used in the analysis of intracellular and extracellular metabolites. Despite the technological advances that allow for the production of high-quality data, the sampling procedures of cultured cells are less well standardized. Different cell lines and culture media composition require adjustments of the protocols to result meaningful quantitative information. Here we provide the workflow for obtaining quantitative metabolic data from adherent mammalian cells using NMR spectroscopy. The robustness of NMR allows for the implementation of the here described protocol to other cell types with only minor adjustments. |
Mendeley readers
The data shown below were compiled from readership statistics for 10 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 10 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Postgraduate | 2 | 20% |
Researcher | 2 | 20% |
Other | 1 | 10% |
Student > Ph. D. Student | 1 | 10% |
Student > Bachelor | 1 | 10% |
Other | 2 | 20% |
Unknown | 1 | 10% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 3 | 30% |
Chemistry | 3 | 30% |
Computer Science | 1 | 10% |
Medicine and Dentistry | 1 | 10% |
Immunology and Microbiology | 1 | 10% |
Other | 0 | 0% |
Unknown | 1 | 10% |