Chapter title |
Analysis of pollutant-induced changes in mitochondrial DNA methylation.
|
---|---|
Chapter number | 19 |
Book title |
Mitochondrial Medicine
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2288-8_19 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2287-1, 978-1-4939-2288-8
|
Authors |
Hyang-Min Byun, Timothy M Barrow, Timothy M. Barrow, Byun, Hyang-Min, Barrow, Timothy M. |
Abstract |
There is increasing evidence that exposure to air pollutants is associated with human disease and may act through epigenetic modification of the nuclear genome, but there have been few publications describing their impact upon the mitochondrial genome. Mitochondrial DNA may be more susceptible to pollutant-induced changes via increased oxidative stress in the cell, and therefore this field of research is of growing interest. Many techniques employed to study DNA methylation of the nuclear genome are also applicable to mitochondrial epigenetic studies. In this chapter, we describe a protocol for the isolation of mitochondrial DNA from peripheral blood samples and the analysis of 5-methylcytosine content by bisulfite pyrosequencing. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 33% |
France | 1 | 33% |
United States | 1 | 33% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 67% |
Science communicators (journalists, bloggers, editors) | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | 4% |
Italy | 1 | 4% |
Unknown | 23 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 8 | 32% |
Student > Master | 3 | 12% |
Student > Bachelor | 3 | 12% |
Researcher | 3 | 12% |
Other | 2 | 8% |
Other | 1 | 4% |
Unknown | 5 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 20% |
Agricultural and Biological Sciences | 5 | 20% |
Medicine and Dentistry | 4 | 16% |
Environmental Science | 2 | 8% |
Chemical Engineering | 1 | 4% |
Other | 2 | 8% |
Unknown | 6 | 24% |