Chapter title |
In Vivo Visualization and Quantification of Mitochondrial Morphology in C. elegans
|
---|---|
Chapter number | 26 |
Book title |
Mitochondrial Medicine
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2288-8_26 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2287-1, 978-1-4939-2288-8
|
Authors |
Smith, Reuben L., De Vos, Winnok H., de Boer, Richard, Manders, Erik M. M., van der Spek, Hans, Reuben L. Smith, Winnok H. De Vos, Richard de Boer, Erik M. M. Manders, Hans van der Spek, Vos, Winnok H. De, Boer, Richard de, Spek, Hans van der |
Abstract |
Caenorhabditis elegans is a highly malleable model system, intensively used for functional, genetic, cytometric, and integrative studies. Due to its simplicity and large muscle cell number, C. elegans has frequently been used to study mitochondrial deficiencies caused by disease or drug toxicity. Here, we describe a robust and efficient method to visualize and quantify mitochondrial morphology in vivo. This method has many practical and technical advantages above traditional (manual) methods and provides a comprehensive analysis of mitochondrial morphology. |
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