Chapter title |
Mass spectrometric investigations of human milk oligosaccharides.
|
---|---|
Chapter number | 35 |
Book title |
Bioactive Components of Human Milk
|
Published in |
Advances in experimental medicine and biology, January 2001
|
DOI | 10.1007/978-1-4615-1371-1_35 |
Pubmed ID | |
Book ISBNs |
978-1-4613-5521-2, 978-1-4615-1371-1
|
Authors |
Pfenninger, A, Karas, M, Finke, B, Stahl, B, Sawatzki, G, Anja Pfenninger, M. Karas, B. Finke, B. Stahl, G. Sawatzki |
Abstract |
Human milk oligosaccharides (OS) have been fractionated by gel permeation chromatography (GPC) and analyzed by mass spectrometry (MS). Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF/MS) and electrospray ionization (ESI) ion trap/MS were used. Using a large human milk pool, the MALDI-TOF/MS analysis of high-molecular-mass GPC fractions showed that complex, multiply fucosylated and/or sialylated OS are present over a larger mass range than described previously Acidic oligosaccharides could be detected from low-retention-time GPC fractions with masses up to 4000 Da, which has not been reported before. |
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