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Plant Programmed Cell Death

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Cover of 'Plant Programmed Cell Death'

Table of Contents

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    Book Overview
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    Chapter 1 Programmed Cell Death in Plants: An Overview
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    Chapter 2 Investigation of Morphological Features of Autophagy During Plant Programmed Cell Death
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    Chapter 3 Markers of Developmentally Regulated Programmed Cell Death and Their Analysis in Cereal Seeds
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    Chapter 4 Measurement of Hypersensitive Cell Death Triggered by Avirulent Bacterial Pathogens in Arabidopsis
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    Chapter 5 Immunity-Associated Programmed Cell Death as a Tool for the Identification of Genes Essential for Plant Innate Immunity
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    Chapter 6 Analysis of Mitochondrial Markers of Programmed Cell Death
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    Chapter 7 Studying Retrograde Signaling in Plants
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    Chapter 8 ROS and Cell Death in Tomato Roots Infected by Meloidogyne Incognita
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    Chapter 9 Detection of Reactive Oxygen and Nitrogen Species (ROS/RNS) During Hypersensitive Cell Death
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    Chapter 10 DNA Diffusion Assay Applied to Plant Cells
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    Chapter 11 Analysis of Reactive Carbonyl Species Generated Under Oxidative Stress
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    Chapter 12 In Vivo Analysis of Calcium Levels and Glutathione Redox Status in Arabidopsis Epidermal Leaf Cells Infected with the Hypersensitive Response-Inducing Bacteria Pseudomonas syringae pv. tomato AvrB (PstAvrB)
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    Chapter 13 Measurement of Cyclic GMP During Plant Hypersensitive Disease Resistance Response
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    Chapter 14 Detection of MAPK3/6 Phosphorylation During Hypersensitive Response (HR)-Associated Programmed Cell Death in Plants
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    Chapter 15 Measurement of the Caspase-1-Like Activity of Vacuolar Processing Enzyme in Plants
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    Chapter 16 Plant Cell Cultures as Model Systems to Study Programmed Cell Death
Attention for Chapter 2: Investigation of Morphological Features of Autophagy During Plant Programmed Cell Death
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Chapter title
Investigation of Morphological Features of Autophagy During Plant Programmed Cell Death
Chapter number 2
Book title
Plant Programmed Cell Death
Published in
Methods in molecular biology, January 2018
DOI 10.1007/978-1-4939-7668-3_2
Pubmed ID
Book ISBNs
978-1-4939-7667-6, 978-1-4939-7668-3
Authors

Alessio Papini

Abstract

The investigation of autophagy particularly when observed during programmed cell death (PCD) is strongly based on the morphological features recorded with transmission electron microscope (TEM). Here we describe methods to induce and to inhibit autophagy in plants. Also some tips for obtaining better preservation of biological membranes, crucial for the investigation of autophagy, are provided together with information about plant autophagic mutants, use of antibodies and methods for 3D reconstruction of large membrane-bound objects that are commonly formed during autophagic processes leading to PCD in plants.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 14 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 3 21%
Other 2 14%
Student > Master 2 14%
Student > Ph. D. Student 2 14%
Student > Bachelor 1 7%
Other 2 14%
Unknown 2 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 5 36%
Biochemistry, Genetics and Molecular Biology 2 14%
Chemical Engineering 1 7%
Earth and Planetary Sciences 1 7%
Engineering 1 7%
Other 0 0%
Unknown 4 29%