| Chapter title |
Measurement of Cyclic GMP During Plant Hypersensitive Disease Resistance Response
|
|---|---|
| Chapter number | 13 |
| Book title |
Plant Programmed Cell Death
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7668-3_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7667-6, 978-1-4939-7668-3
|
| Authors |
Jian Chen, Diana Bellin, Elodie Vandelle |
| Abstract |
Cyclic guanosine-3',5'-monophosphate (cGMP) is recognized as an important second messenger in plants, mediating intracellular signal in important physiological processes, including the hypersensitive disease resistance response induced by avirulent pathogens. In this context, the analysis of cGMP levels in infected plants requires an accurate and specific detection method allowing its quantification. Here, we describe an assay based on the Alphascreen technology, developed for animal cells and further adapted and optimized for the detection of cGMP in plants. The method is applied for the measurement of cGMP in Arabidopsis thaliana plants challenged with an avirulent strain of Pseudomonas syringae pv. tomato. This protocol includes the extraction of cGMP, the assay procedure and the calculation of cGMP concentration. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 2 | 40% |
| Other | 1 | 20% |
| Unspecified | 1 | 20% |
| Unknown | 1 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 2 | 40% |
| Biochemistry, Genetics and Molecular Biology | 1 | 20% |
| Unspecified | 1 | 20% |
| Unknown | 1 | 20% |