| Chapter title |
Peptide mass fingerprinting: protein identification using MALDI-TOF mass spectrometry.
|
|---|---|
| Chapter number | 16 |
| Book title |
Chemical Genomics
|
| Published in |
Methods in molecular biology, December 2005
|
| DOI | 10.1007/978-1-59259-948-6_16 |
| Pubmed ID | |
| Book ISBNs |
978-1-58829-399-2, 978-1-59259-948-6
|
| Authors |
Webster J, Oxley D, Judith Webster, David Oxley, Webster, Judith, Oxley, David |
| Abstract |
Matrix-assisted laser desorption/ionization (MALDI)-time-of-flight (TOF)-mass spectrometry (MS) is now routinely used in many laboratories for the rapid and sensitive identification of proteins by peptide mass fingerprinting (PMF). We describe a simple protocol that can be performed in a standard biochemistry laboratory, whereby proteins separated by one- or two-dimensional gel electrophoresis can be identified at femtomole levels. The procedure involves excision of the spot or band from the gel, washing and de-staining, reduction and alkylation, in-gel trypsin digestion, MALDI-TOF MS of the tryptic peptides, and database searching of the PMF data. Up to 96 protein samples can easily be manually processed at one time by this method. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Russia | 1 | 4% |
| Unknown | 23 | 96% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 6 | 25% |
| Student > Ph. D. Student | 6 | 25% |
| Researcher | 4 | 17% |
| Student > Doctoral Student | 2 | 8% |
| Student > Master | 2 | 8% |
| Other | 2 | 8% |
| Unknown | 2 | 8% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 8 | 33% |
| Biochemistry, Genetics and Molecular Biology | 5 | 21% |
| Chemistry | 3 | 13% |
| Engineering | 2 | 8% |
| Linguistics | 1 | 4% |
| Other | 3 | 13% |
| Unknown | 2 | 8% |