Chapter title |
Vitrification of heart valve tissues.
|
---|---|
Chapter number | 20 |
Book title |
Cryopreservation and Freeze-Drying Protocols
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2193-5_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2192-8, 978-1-4939-2193-5
|
Authors |
Kelvin G M Brockbank, Zhenzhen Chen, Elizabeth D Greene, Lia H Campbell, Kelvin G. M. Brockbank, Elizabeth D. Greene, Lia H. Campbell, Brockbank, Kelvin G. M., Chen, Zhenzhen, Greene, Elizabeth D., Campbell, Lia H. |
Abstract |
Application of the original vitrification protocol used for pieces of heart valves to intact heart valves has evolved over time. Ice-free cryopreservation by Protocol 1 using VS55 is limited to small samples where relatively rapid cooling and warming rates are possible. VS55 cryopreservation typically provides extracellular matrix preservation with approximately 80 % cell viability and tissue function compared with fresh untreated tissues. In contrast, ice-free cryopreservation using VS83, Protocols 2 and 3, has several advantages over conventional cryopreservation methods and VS55 preservation, including long-term preservation capability at -80 °C; better matrix preservation than freezing with retention of material properties; very low cell viability, reducing the risks of an immune reaction in vivo; reduced risks of microbial contamination associated with use of liquid nitrogen; improved in vivo functions; no significant recipient allogeneic immune response; simplified manufacturing process; increased operator safety because liquid nitrogen is not used; and reduced manufacturing costs. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 30 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 6 | 20% |
Student > Bachelor | 4 | 13% |
Researcher | 4 | 13% |
Lecturer | 2 | 7% |
Student > Ph. D. Student | 2 | 7% |
Other | 4 | 13% |
Unknown | 8 | 27% |
Readers by discipline | Count | As % |
---|---|---|
Engineering | 7 | 23% |
Biochemistry, Genetics and Molecular Biology | 6 | 20% |
Agricultural and Biological Sciences | 5 | 17% |
Materials Science | 2 | 7% |
Nursing and Health Professions | 1 | 3% |
Other | 1 | 3% |
Unknown | 8 | 27% |