Chapter title |
Application of shotgun proteomics for discovery-driven protein-protein interaction.
|
---|---|
Chapter number | 18 |
Book title |
Shotgun Proteomics
|
Published in |
Methods in molecular biology, April 2014
|
DOI | 10.1007/978-1-4939-0685-7_18 |
Pubmed ID | |
Book ISBNs |
978-1-4939-0684-0, 978-1-4939-0685-7
|
Authors |
Goto-Silva L, Maliga Z, Slabicki M, Murillo JR, Junqueira M, Livia Goto-Silva, Zoltan Maliga, Mikolaj Slabicki, Jimmy Rodriguez Murillo, Magno Junqueira, Goto-Silva, Livia, Maliga, Zoltan, Slabicki, Mikolaj, Murillo, Jimmy Rodriguez, Junqueira, Magno |
Editors |
Daniel Martins-de-Souza |
Abstract |
Affinity purification of protein complexes and identification of co-purified proteins by mass spectrometry is a powerful method to discover novel protein-protein interactions. Application of this method to the study of biological systems often requires the ability to process a large number of samples. Hence, there is great need to generate proteomic workflows compatible with large-scale studies. The major goal of this protocol is to present a fast, reliable, and scalable method to characterize protein complexes by mass spectrometry to overcome the limitations of conventional geLC-MS/MS or MudPIT protocols. This method was successfully employed for the discovery and characterization of novel protein complexes in cultured yeast, mammalian cells, and mice. |
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Members of the public | 1 | 100% |
Mendeley readers
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Unknown | 6 | 100% |
Demographic breakdown
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Student > Master | 2 | 33% |
Unspecified | 1 | 17% |
Researcher | 1 | 17% |
Student > Ph. D. Student | 1 | 17% |
Unknown | 1 | 17% |
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Agricultural and Biological Sciences | 3 | 50% |
Computer Science | 1 | 17% |
Unspecified | 1 | 17% |
Unknown | 1 | 17% |