Chapter title |
Survey of shotgun proteomics.
|
---|---|
Chapter number | 1 |
Book title |
Shotgun Proteomics
|
Published in |
Methods in molecular biology, April 2014
|
DOI | 10.1007/978-1-4939-0685-7_1 |
Pubmed ID | |
Book ISBNs |
978-1-4939-0684-0, 978-1-4939-0685-7
|
Authors |
Fabio Cesar Sousa Nogueira, Gilberto B. Domont, Nogueira, Fabio Cesar Sousa, Domont, Gilberto B. |
Editors |
Daniel Martins-de-Souza |
Abstract |
Proteins provide the verbs to biology, and proteomics provides the nouns for their analytical and discovery-driven studies. The term proteomics was coined in the 1990s and deals with the protein complement of the genome-the proteome. Following the classical proteomics era, the development of new mass spectrometric methods for peptide analysis permitted the identification of proteins in peptide mixtures obtained by proteolytic digestion of complex samples, e.g., shotgun proteomics. Since its introduction, shotgun proteomics became the standard technique for the analysis of protein hydrolyzates in a high-throughput way. In this chapter, we provide a survey in shotgun proteomics highlighting instruments and techniques used in modern second and third proteomics generation. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 50% |
Scientists | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 50 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 13 | 26% |
Researcher | 10 | 20% |
Student > Master | 6 | 12% |
Professor > Associate Professor | 3 | 6% |
Student > Bachelor | 2 | 4% |
Other | 10 | 20% |
Unknown | 6 | 12% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 14 | 28% |
Biochemistry, Genetics and Molecular Biology | 12 | 24% |
Medicine and Dentistry | 5 | 10% |
Chemistry | 4 | 8% |
Business, Management and Accounting | 1 | 2% |
Other | 6 | 12% |
Unknown | 8 | 16% |