Chapter title |
Techniques for Experimental Infection of Mosquitoes with West Nile Virus
|
---|---|
Chapter number | 13 |
Book title |
West Nile Virus
|
Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3670-0_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3668-7, 978-1-4939-3670-0
|
Authors |
Yang Liu, Gong Cheng |
Abstract |
West Nile virus is a typical mosquito-borne flavivirus, and it is transmitted between mosquitoes and birds in nature. As an incidental host, humans are susceptible to WNV infection. WNV infection in humans can result in fever, meningitis, and encephalitis. Approved human vaccines or therapies are not available for WNV infection. In this chapter, we focus on the techniques for WNV infection and detection in mosquitoes. The technical details include: (1) WNV infection in cell culture; (2) Mosquito rearing; (3) WNV infection in mosquitoes via thoracic microinjection; (4) Detection of WNV infection in mosquitoes; (5) Determination of WNV M.I.D50 in mosquitoes; (6) WNV infection in mosquitoes via membrane blood feeding; (7) WNV infection via blood feeding on mice; (8) Immunofluorescence staining of WNV infected mosquito tissues. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 6% |
Unknown | 17 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 5 | 28% |
Other | 3 | 17% |
Student > Doctoral Student | 2 | 11% |
Student > Bachelor | 2 | 11% |
Student > Ph. D. Student | 1 | 6% |
Other | 2 | 11% |
Unknown | 3 | 17% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 9 | 50% |
Veterinary Science and Veterinary Medicine | 2 | 11% |
Medicine and Dentistry | 2 | 11% |
Immunology and Microbiology | 1 | 6% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 6% |
Other | 0 | 0% |
Unknown | 3 | 17% |