Chapter title |
Examination of West Nile Virus Neuroinvasion and Neuropathogenesis in the Central Nervous System of a Murine Model
|
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Chapter number | 8 |
Book title |
West Nile Virus
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Published in |
Methods in molecular biology, January 2016
|
DOI | 10.1007/978-1-4939-3670-0_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3668-7, 978-1-4939-3670-0
|
Authors |
Hameeda Sultana |
Abstract |
West Nile virus (WNV) is a neurotropic virus that causes inflammation and neuronal loss in the Central Nervous System leading to encephalitis and death. In this chapter, detailed methods to detect WNV in the murine brain tissue by quantitative real-time polymerase chain reaction and viral plaque assays are described. Determination of WNV neuropathogenesis by Hematoxylin and Eosin staining and immunohistochemical procedures are provided. In addition, TUNEL assays to determine neuronal loss during WNV neuropathogenesis are discussed in detail. Collectively, the methods mentioned in this chapter provide an overview to understand neuroinvasion and neuropathogenesis in a murine model of WNV infection. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 4 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 1 | 25% |
Professor > Associate Professor | 1 | 25% |
Researcher | 1 | 25% |
Student > Doctoral Student | 1 | 25% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 2 | 50% |
Biochemistry, Genetics and Molecular Biology | 1 | 25% |
Unknown | 1 | 25% |