| Chapter title |
Enhancing Human Cardiomyocyte Differentiation from Induced Pluripotent Stem Cells with Trichostatin A.
|
|---|---|
| Chapter number | 160 |
| Book title |
Induced Pluripotent Stem (iPS) Cells
|
| Published in |
Methods in molecular biology, December 2014
|
| DOI | 10.1007/7651_2014_160 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3054-8, 978-1-4939-3055-5
|
| Authors |
Shiang Y Lim, Priyadharshini Sivakumaran, Duncan E Crombie, Gregory J Dusting, Alice Pébay, Rodney J Dilley, Lim SY, Sivakumaran P, Crombie DE, Dusting GJ, Pébay A, Dilley RJ, Shiang Y. Lim Ph.D., Duncan E. Crombie, Gregory J. Dusting, Rodney J. Dilley Ph.D., Kursad Turksen, Andras Nagy, Shiang Y. Lim, Rodney J. Dilley, Lim, Shiang Y., Sivakumaran, Priyadharshini, Crombie, Duncan E., Dusting, Gregory J., Pébay, Alice, Dilley, Rodney J. |
| Editors |
Kursad Turksen, Andras Nagy |
| Abstract |
Human induced pluripotent stem (iPS) cells are a promising source of autologous cardiomyocytes to repair and regenerate myocardium for treatment of heart disease. In this study, we describe a method for enhanced cardiomyocyte production from human iPS cells by treating embryoid bodies with a histone deacetylase inhibitor, trichostatin A (TSA), together with activin A and bone morphogenetic protein (BMP)-4. The resulting cardiomyocytes expressed cardiac-specific transcription factors and contractile proteins at both gene and protein levels. Functionally, the contractile embryoid bodies (EBs) displayed calcium cycling and were responsive to the chronotropic agents isoprenaline (0.1 μM) and carbachol (1 μM). The cardiomyocytes derived from human iPS cells may be used to engineer functional cardiac muscle tissue for studying pathophysiology of cardiac disease, for drug discovery test beds, and potentially for generation of cardiac grafts to surgically replace damaged myocardium. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 19 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 5 | 26% |
| Student > Bachelor | 2 | 11% |
| Unspecified | 1 | 5% |
| Librarian | 1 | 5% |
| Student > Master | 1 | 5% |
| Other | 2 | 11% |
| Unknown | 7 | 37% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 4 | 21% |
| Biochemistry, Genetics and Molecular Biology | 3 | 16% |
| Unspecified | 1 | 5% |
| Social Sciences | 1 | 5% |
| Engineering | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 9 | 47% |