Chapter title |
Sendai Virus-Based Reprogramming of Mesenchymal Stromal/Stem Cells from Umbilical Cord Wharton's Jelly into Induced Pluripotent Stem Cells.
|
---|---|
Chapter number | 163 |
Book title |
Induced Pluripotent Stem (iPS) Cells
|
Published in |
Methods in molecular biology, August 2015
|
DOI | 10.1007/7651_2014_163 |
Pubmed ID | |
Book ISBNs |
978-1-4939-3054-8, 978-1-4939-3055-5
|
Authors |
Cristian Miere, Liani Devito, Dusko Ilic, Miere, Cristian, Devito, Liani, Ilic, Dusko |
Editors |
Kursad Turksen, Andras Nagy |
Abstract |
In an attempt to bring pluripotent stem cell biology closer to reaching its full potential, many groups have focused on improving reprogramming protocols over the past several years. The episomal modified Sendai virus-based vector has emerged as one of the most practical ones. Here we describe reprogramming of mesenchymal stromal/stem cells (MSC) derived from umbilical cord Wharton's Jelly into induced pluripotent stem cells (iPSC) using genome non-integrating Sendai virus-based vectors. The detailed protocols of iPSC colony cryopreservation (vitrification) and adaption to feeder-free culture conditions are also included. |
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Mendeley readers
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