Chapter title |
In vivo assessment of neuronal cell death in Drosophila Drosophila.
|
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Chapter number | 25 |
Book title |
Neuronal Cell Death
|
Published in |
Methods in molecular biology, January 2015
|
DOI | 10.1007/978-1-4939-2152-2_25 |
Pubmed ID | |
Book ISBNs |
978-1-4939-2151-5, 978-1-4939-2152-2
|
Authors |
Pierre Dourlen, Dourlen, Pierre |
Abstract |
Following neuronal cell deathcell death at the cellular level and over several time points is challenging in living animal because of the difficulty of accessing and identifying individual neuronsneurons . In the eyeeye of a living Drosophila Drosophila , it is possible to visualize neurons thanks to the corneacornea neutralizcornea neutralization technique ation technique. This technique can be coupled to the generation of mosaic clonesmosaic clones by the TomatoTomato /GFPGFP -FLP/FRTFRT methodTomato/GFP-FLP/FRT method to identify a group of photoreceptorphotoreceptor neurons at a single-cell resolutionsingle-cell resolution . This method has proved to be efficient for the study of photoreceptor developmentdevelopment and degenerationdegeneration . In this chapter, I describe this method and focus on fatp mutant fatp mutant photoreceptor neuron degeneration. |
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