Chapter title |
Avian influenza virus detection and quantitation by real-time rt-PCR.
|
---|---|
Chapter number | 10 |
Book title |
Animal Influenza Virus
|
Published in |
Methods in molecular biology, June 2014
|
DOI | 10.1007/978-1-4939-0758-8_10 |
Pubmed ID | |
Book ISBNs |
978-1-4939-0757-1, 978-1-4939-0758-8
|
Authors |
Spackman E, Erica Spackman |
Abstract |
Real-time RT-PCR (rRT-PCR) has been used for avian influenza virus (AIV) detection since the early 2000s for routine surveillance, outbreak assessment and for research. Some of the advantages of rRT-PCR are: high sensitivity, high specificity, rapid time-to-result, scalability, cost, and its inherently quantitative nature. Furthermore, rRT-PCR can be used with numerous sample types, is less expensive than virus isolation in chicken embryos, and since infectious virus is inactivated early during processing, biosafety and bio-security are also easier to maintain. However, the high genetic variability of AIV may decrease sensitivity and increases the chances of a false negative result. This chapter provides an overview of the USDA validated rRT-PCR procedure for the detection of type A influenza. |
X Demographics
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United Kingdom | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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Hong Kong | 1 | 6% |
Unknown | 15 | 94% |
Demographic breakdown
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Student > Master | 4 | 25% |
Student > Ph. D. Student | 3 | 19% |
Researcher | 3 | 19% |
Professor | 2 | 13% |
Unknown | 4 | 25% |
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Biochemistry, Genetics and Molecular Biology | 2 | 13% |
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Earth and Planetary Sciences | 1 | 6% |
Other | 2 | 13% |
Unknown | 3 | 19% |