Chapter title |
Enzymatic Assays to Investigate Acyl-Homoserine Lactone Autoinducer Synthases
|
---|---|
Chapter number | 13 |
Book title |
Quorum Sensing
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7309-5_13 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7308-8, 978-1-4939-7309-5
|
Authors |
Daniel Shin, Rajesh Nagarajan |
Abstract |
Bacteria use chemical molecules called autoinducers as votes to poll their numerical strength in a colony. This polling mechanism, commonly referred to as quorum sensing, enables bacteria to build a social network and provide a collective response for fighting off common threats. In Gram-negative bacteria, AHL synthases synthesize acyl-homoserine lactone (AHL) autoinducers to turn on the expression of several virulent genes including biofilm formation, protease secretion, and toxin production. Therefore, inhibiting AHL signal synthase would limit quorum sensing and virulence. In this chapter, we describe four enzymatic methods that could be adopted to investigate a broad array of AHL synthases. The enzymatic assays described here should accelerate our mechanistic understanding of quorum-sensing signal synthesis that could pave the way for discovery of potent antivirulence compounds. |
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