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Chapter title |
Ultra-Performance Liquid Chromatography/Mass Spectrometry for the Detection and Quantification of Diffusible Signal Factor (DSF) Family Quorum-Sensing Signals
|
---|---|
Chapter number | 8 |
Book title |
Quorum Sensing
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-7309-5_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7308-8, 978-1-4939-7309-5
|
Authors |
Lian Zhou, Shuang Sun, Wei Zhang, Ya-Wen He |
Abstract |
Molecules of the diffusible signal factor (DSF) family represent a class of widely conserved quorum-sensing signals used by many Gram-negative bacterial pathogens. The measurement of DSF family signals is essential for understanding their biological roles, signaling pathway, and regulatory network. We are presenting here methods for extraction and purification of DSF family signals from culture supernatants, and further quantification of members of DSF family signals using an ultra-performance liquid chromatographic system (UPLC) coupled with an accurate time-of-flight mass spectrometry (TOF-MS) analysis. |
Mendeley readers
The data shown below were compiled from readership statistics for 5 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 2 | 40% |
Unspecified | 1 | 20% |
Student > Ph. D. Student | 1 | 20% |
Student > Doctoral Student | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Arts and Humanities | 1 | 20% |
Unspecified | 1 | 20% |
Biochemistry, Genetics and Molecular Biology | 1 | 20% |
Business, Management and Accounting | 1 | 20% |
Unknown | 1 | 20% |