Chapter title |
Fluorescence Quenching Studies of γ-Butyrolactone-Binding Protein (CprB) from Streptomyces coelicolor A3(2)
|
---|---|
Chapter number | 11 |
Book title |
Quorum Sensing
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7309-5_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7308-8, 978-1-4939-7309-5
|
Authors |
Jessy Mariam, Ruchi Anand |
Abstract |
Fluorescence spectroscopy is an important analytical tool which is widely employed to study biological systems. This technique can be applied to qualitatively and quantitatively probe protein-ligand interactions primarily because of its sensitivity, selectivity, nondestructive and rapid form of analysis. In this chapter we describe the utility of this technique to establish a label-free, universal screening protocol for putative γ-butyrolactone (GBL) receptors by exploiting the intrinsic fluorescence of a highly conserved tryptophan residue that constitutes the hydrophobic pocket for GBL binding, a unique feature possessed by this family of receptors. Here we demonstrate this technique using a combination of steady-state fluorescence quenching methods and fluorescence lifetime decay kinetics using CprB protein from Streptomyces coelicolor A3(2) as a model system. Interaction data between CprB and two chemically synthesized GBLs involved in quorum sensing, Cp1 and Cp2, have been used as example. |
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