Chapter title |
Construction and Selection of Affilin® Phage Display Libraries
|
---|---|
Chapter number | 11 |
Book title |
Phage Display
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7447-4_11 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7446-7, 978-1-4939-7447-4
|
Authors |
Florian Settele, Madlen Zwarg, Sebastian Fiedler, Daniel Koscheinz, Eva Bosse-Doenecke, Settele, Florian, Zwarg, Madlen, Fiedler, Sebastian, Koscheinz, Daniel, Bosse-Doenecke, Eva |
Abstract |
Affilin(®) molecules represent a new class of so-called scaffold proteins. The concept of scaffold proteins is to use stable and versatile protein structures which can be endowed with de novo binding properties and specificities by introducing mutations in surface exposed amino acid residues. Complex variations and combinations are generated by genetic methods of randomization resulting in large cDNA libraries. The selection for candidates binding to a desired target can be executed by display methods, especially the very robust and flexible phage display. Here, we describe the construction of ubiquitin based Affilin(®) phage display libraries and their use in biopanning experiments for the identification of novel protein ligands. |
Mendeley readers
Geographical breakdown
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Unknown | 9 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 1 | 11% |
Researcher | 1 | 11% |
Student > Master | 1 | 11% |
Unknown | 5 | 56% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 1 | 11% |
Unknown | 6 | 67% |