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Mendeley readers
| Chapter title |
Construction of Chicken Antibody Libraries
|
|---|---|
| Chapter number | 10 |
| Book title |
Phage Display
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7447-4_10 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7446-7, 978-1-4939-7447-4
|
| Authors |
Jeanni Fehrsen, Susan Wemmer, Wouter van Wyngaardt |
| Abstract |
Recombinant antibody libraries based on chicken immunoglobulin genes are potentially valuable sources of phage displayed scFvs for use in veterinary diagnostics and research. The libraries described in this chapter are based on chicken variable heavy and light chain immunoglobulin genes joined by a short flexible linker cloned in the phagemid vector pHEN1. The resulting phagemids produce either scFvs displayed on the surface of the fusion phage subsequent to co-infection with helper phage, or soluble scFvs following IPTG induction. This chapter provides detailed and proven methods for the construction of such libraries. |
Mendeley readers
The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 11 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 3 | 27% |
| Student > Doctoral Student | 2 | 18% |
| Researcher | 2 | 18% |
| Student > Ph. D. Student | 1 | 9% |
| Other | 1 | 9% |
| Other | 1 | 9% |
| Unknown | 1 | 9% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 55% |
| Veterinary Science and Veterinary Medicine | 1 | 9% |
| Agricultural and Biological Sciences | 1 | 9% |
| Earth and Planetary Sciences | 1 | 9% |
| Medicine and Dentistry | 1 | 9% |
| Other | 0 | 0% |
| Unknown | 1 | 9% |