Chapter title |
Generation of Semi-Synthetic Shark IgNAR Single-Domain Antibody Libraries
|
---|---|
Chapter number | 8 |
Book title |
Phage Display
|
Published in |
Methods in molecular biology, January 2018
|
DOI | 10.1007/978-1-4939-7447-4_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7446-7, 978-1-4939-7447-4
|
Authors |
Grzeschik, Julius, Könning, Doreen, Hinz, Steffen C, Krah, Simon, Schröter, Christian, Empting, Martin, Kolmar, Harald, Zielonka, Stefan, Julius Grzeschik, Doreen Könning, Steffen C. Hinz, Simon Krah, Christian Schröter, Martin Empting, Harald Kolmar, Stefan Zielonka |
Abstract |
Besides classical antibodies with the composition of heavy and light chains, sharks produce a unique heavy chain only isotype, termed Immunoglobulin New Antigen Receptor (IgNAR), in which antigen binding is solely mediated by a single domain, referred to as vNAR. Owing to their high affinity and specificity combined with their small size and high stability, vNAR domains emerged as promising target-binding scaffolds that can be tailor-made for biotechnological and biomedical applications. Herein, we describe protocols for the construction of semi-synthetic, CDR3-randomized vNAR libraries for the isolation of target-specific antibodies using yeast surface display or phage display as platform technology. Additionally, we provide information for affinity maturation of target-specific molecules through CDR1 diversification and sublibrary establishment. |
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Members of the public | 1 | 100% |
Mendeley readers
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Demographic breakdown
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Student > Bachelor | 3 | 21% |
Researcher | 2 | 14% |
Student > Ph. D. Student | 1 | 7% |
Student > Doctoral Student | 1 | 7% |
Student > Master | 1 | 7% |
Other | 1 | 7% |
Unknown | 5 | 36% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 4 | 29% |
Veterinary Science and Veterinary Medicine | 2 | 14% |
Agricultural and Biological Sciences | 2 | 14% |
Chemistry | 1 | 7% |
Unknown | 5 | 36% |