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Mendeley readers
Chapter title |
Nucleic Acid-Binding Assay of Argonaute Protein Using Fluorescence Polarization
|
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Chapter number | 8 |
Book title |
Argonaute Proteins
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Published in |
Methods in molecular biology, January 2018
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DOI | 10.1007/978-1-4939-7339-2_8 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7338-5, 978-1-4939-7339-2
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Authors |
Tomohiro Miyoshi |
Abstract |
Nucleic acid binding by the Argonaute protein is an important trigger step in the Argonaute-dependent gene silencing system. We established an in vitro method to detect the nucleic acid binding activity of the Argonaute protein by fluorescence polarization. In this chapter, we will describe the expression and purification of the prokaryotic (Rhodobacter sphaeroides) Argonaute protein, and the nucleic acid-binding analysis using a Fluorescence Polarization System (Beacon 2000). |
Mendeley readers
The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 6 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 2 | 33% |
Student > Bachelor | 1 | 17% |
Student > Doctoral Student | 1 | 17% |
Researcher | 1 | 17% |
Unknown | 1 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 4 | 67% |
Agricultural and Biological Sciences | 1 | 17% |
Unknown | 1 | 17% |