Chapter title |
Mapping the genomic binding sites of the activated retinoid x receptor in murine bone marrow-derived macrophages using chromatin immunoprecipitation sequencing.
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Chapter number | 2 |
Book title |
Steroid Receptors
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Published in |
Methods in molecular biology, January 2014
|
DOI | 10.1007/978-1-4939-1346-6_2 |
Pubmed ID | |
Book ISBNs |
978-1-4939-1345-9, 978-1-4939-1346-6
|
Authors |
Bence Daniel, Balint L Balint, Zsuzsanna S Nagy, Laszlo Nagy, Balint L. Balint, Zsuzsanna S. Nagy |
Abstract |
Chromatin immunoprecipitation followed by massively parallel sequencing (ChIP-Seq) is a powerful technique to map the genomic location of a given chromatin bound factor (i.e., transcription factors, cofactors) or epigenetic marks, such as histone modification. The procedure is based on cross-linking of proteins to DNA followed by the capture of the protein-DNA complexes by "ChIP-grade" antibodies. In this chapter we describe in detail the experimental method developed in our laboratory to investigate in vivo the DNA-binding characteristics of a key heterodimeric nuclear receptor, the retinoid X receptor (RXR) in murine bone marrow-derived macrophages. |
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