| Chapter title |
Isolation of MicroRNAs that Regulate Seed Dormancy and Germination.
|
|---|---|
| Chapter number | 13 |
| Book title |
Seed Dormancy
|
| Published in |
Methods in molecular biology, January 2011
|
| DOI | 10.1007/978-1-61779-231-1_13 |
| Pubmed ID | |
| Book ISBNs |
978-1-61779-230-4, 978-1-61779-231-1
|
| Authors |
M. B. Arun Kumar, Ruth C. Martin, Hiro Nonogaki, Kumar, M. B. Arun, Martin, Ruth C., Nonogaki, Hiro |
| Abstract |
MicroRNAs (miRNAs) play an important role in gene regulation in many plant tissues and organs during various developmental stages. Previous studies have suggested the importance of gene regulation by miRNA in seeds. Characterizing the expression of miRNAs and their target genes in dormant and germinating seeds helps to gain a better understanding of the regulatory role of miRNAs during seed dormancy and germination. This can be achieved by implementing a simple miRNA extraction method using fractionation with isopropanol and Northern blot analysis using nonradioactive miRNA probes. Functional analysis of miRNA target genes potentially associated with seed dormancy and germination can be examined using mutant seeds in which specific miRNAs are deregulated by introducing silent mutations in the miRNA target sites of these genes. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Australia | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 23% |
| Student > Ph. D. Student | 2 | 15% |
| Professor > Associate Professor | 2 | 15% |
| Student > Master | 1 | 8% |
| Other | 1 | 8% |
| Other | 2 | 15% |
| Unknown | 2 | 15% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 8 | 62% |
| Biochemistry, Genetics and Molecular Biology | 2 | 15% |
| Chemical Engineering | 1 | 8% |
| Unknown | 2 | 15% |