| Chapter title |
Sequential ChIP Protocol for Profiling Bivalent Epigenetic Modifications (ReChIP)
|
|---|---|
| Chapter number | 6 |
| Book title |
Plant Chromatin Dynamics
|
| Published in |
Methods in molecular biology, January 2018
|
| DOI | 10.1007/978-1-4939-7318-7_6 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-7317-0, 978-1-4939-7318-7
|
| Authors |
Bénédicte Desvoyes, Joana Sequeira-Mendes, Zaida Vergara, Sofia Madeira, Crisanto Gutierrez, Desvoyes, Bénédicte, Sequeira-Mendes, Joana, Vergara, Zaida, Madeira, Sofia, Gutierrez, Crisanto |
| Abstract |
Identification of chromatin modifications, e.g., histone acetylation and methylation, among others, is widely carried out by using a chromatin immunoprecipitation (ChIP) strategy. The information obtained with these procedures is useful to gain an overall picture of modifications present in all cells of the population under study. It also serves as a basis to figure out the mechanisms of chromatin organization and gene regulation at the population level. However, the ultimate goal is to understand gene regulation at the level of single chromatin fibers. This requires the identification of chromatin modifications that occur at a given genomic location and within the same chromatin fiber. This is achieved by following a sequential ChIP strategy using two antibodies to distinguish different chromatin modifications. Here, we describe a sequential ChIP protocol (Re-ChIP), paying special attention to the controls needed and the required steps to obtain meaningful and reproducible results. The protocol is developed for young Arabidopsis seedlings but could be adapted to other plant materials. |
X Demographics
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Spain | 3 | 60% |
| Austria | 1 | 20% |
| United States | 1 | 20% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 4 | 80% |
| Members of the public | 1 | 20% |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 14 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 3 | 21% |
| Student > Master | 3 | 21% |
| Professor | 2 | 14% |
| Student > Doctoral Student | 2 | 14% |
| Student > Ph. D. Student | 2 | 14% |
| Other | 1 | 7% |
| Unknown | 1 | 7% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 6 | 43% |
| Biochemistry, Genetics and Molecular Biology | 4 | 29% |
| Psychology | 1 | 7% |
| Neuroscience | 1 | 7% |
| Engineering | 1 | 7% |
| Other | 0 | 0% |
| Unknown | 1 | 7% |