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Neutrophil Methods and Protocols

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Cover of 'Neutrophil Methods and Protocols'

Table of Contents

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    Book Overview
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    Chapter 1 The Role of Neutrophils in the Immune System: An Overview
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    Chapter 2 Isolation of Human Neutrophils from Venous Blood
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    Chapter 3 Neutrophil Isolation from Nonhuman Species
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    Chapter 4 Collection of In Vivo Transmigrated Neutrophils from Human Skin
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    Chapter 5 Subcellular fractionation of human neutrophils and analysis of subcellular markers.
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    Chapter 6 Neutrophil Methods and Protocols
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    Chapter 7 Measurement of Phospholipid Metabolism in Intact Neutrophils
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    Chapter 8 Optical Methods for the Measurement and Manipulation of Cytosolic Calcium Signals in Neutrophils
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    Chapter 9 Analysis of Electrophysiological Properties and Responses of Neutrophils
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    Chapter 10 Assessment of Neutrophil Apoptosis
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    Chapter 11 Microinjection Methods for Neutrophils
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    Chapter 12 Generation of Functionally Mature Neutrophils from Induced Pluripotent Stem Cells
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    Chapter 13 Neutrophil Methods and Protocols
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    Chapter 14 Spinning disk confocal imaging of neutrophil migration in zebrafish.
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    Chapter 15 Detection of Bidirectional Signaling During Integrin Activation and Neutrophil Adhesion
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    Chapter 16 Immunofluorescence and confocal microscopy of neutrophils.
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    Chapter 17 Expression of genetically encoded fluorescent probes to monitor phospholipid dynamics in live neutrophils.
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    Chapter 18 Quantitative assessment of neutrophil phagocytosis using flow cytometry.
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    Chapter 19 Analysis of neutrophil bactericidal activity.
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    Chapter 20 Induction and quantification of neutrophil extracellular traps.
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    Chapter 21 Measurement of Respiratory Burst Products, Released or Retained, During Activation of Professional Phagocytes
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    Chapter 22 Cell-Free NADPH Oxidase Activation Assays: “In Vitro Veritas”
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    Chapter 23 Assessment of Priming of the Human Neutrophil Respiratory Burst
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    Chapter 24 Affinity purification and reconstitution of human phagocyte flavocytochrome B for detection of conformational dynamics in the membrane.
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    Chapter 25 Evaluation of p47phox Phosphorylation in Human Neutrophils Using Phospho-Specific Antibodies
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    Chapter 26 Genome-Scale Transcript Analyses with Human Neutrophils
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    Chapter 27 Fast and Accurate Quantitative Analysis of Cytokine Gene Expression in Human Neutrophils
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    Chapter 28 High-purity neutrophil isolation from human peripheral blood and saliva for transcriptome analysis.
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    Chapter 29 Detection of intact transcription factors in human neutrophils.
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    Chapter 30 Disorders of neutrophil function: an overview.
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    Chapter 31 Diagnostic Assays for Chronic Granulomatous Disease and Other Neutrophil Disorders
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    Chapter 32 Diagnostic Assays for Myeloperoxidase and Myeloperoxidase Deficiency
Attention for Chapter 30: Disorders of neutrophil function: an overview.
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Chapter title
Disorders of neutrophil function: an overview.
Chapter number 30
Book title
Neutrophil Methods and Protocols
Published in
Methods in molecular biology, February 2014
DOI 10.1007/978-1-62703-845-4_30
Pubmed ID
Book ISBNs
978-1-62703-844-7, 978-1-62703-845-4
Authors

Dinauer MC, Mary C. Dinauer, Dinauer, Mary C.

Abstract

Primary disorders of neutrophil function result from impairment in neutrophil responses that are critical for host defense. This chapter summarizes inherited disorders of neutrophils that cause defects in neutrophil adhesion, migration, and oxidative killing. These include the leukocyte adhesion deficiencies, actin defects, and other disorders of chemotaxis, hyperimmunoglobulin E syndrome, Chédiak-Higashi syndrome, neutrophil-specific granule deficiency, chronic granulomatous disease, and myeloperoxidase deficiency. Diagnostic tests and treatment approaches are also summarized for each neutrophil disorder.

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 71 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Netherlands 1 1%
Unknown 70 99%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 14 20%
Researcher 14 20%
Student > Master 10 14%
Student > Bachelor 8 11%
Student > Doctoral Student 3 4%
Other 11 15%
Unknown 11 15%
Readers by discipline Count As %
Agricultural and Biological Sciences 15 21%
Medicine and Dentistry 14 20%
Biochemistry, Genetics and Molecular Biology 13 18%
Immunology and Microbiology 8 11%
Chemistry 2 3%
Other 4 6%
Unknown 15 21%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 October 2014.
All research outputs
#20,239,689
of 22,766,595 outputs
Outputs from Methods in molecular biology
#9,865
of 13,090 outputs
Outputs of similar age
#268,328
of 310,339 outputs
Outputs of similar age from Methods in molecular biology
#436
of 654 outputs
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So far Altmetric has tracked 13,090 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
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We're also able to compare this research output to 654 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.