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Nuclear Reprogramming

Overview of attention for book
Cover of 'Nuclear Reprogramming'

Table of Contents

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    Book Overview
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    Chapter 1 Nuclear Transfer in the Mouse
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    Chapter 2 Nuclear Transfer in Rabbit
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    Chapter 3 Nuclear Transfer in Ruminants
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    Chapter 4 Nuclear Transfer and Transgenesis in the Pig
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    Chapter 5 Nuclear Reprogramming
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    Chapter 6 Analysis of nuclear reprogramming following nuclear transfer to Xenopus oocyte.
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    Chapter 7 Assessing the Quality of Donor Cells: Karyotyping Methods
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    Chapter 8 Treatment of Donor Cell/Embryo with Different Approaches to Improve Development After Nuclear Transfer
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    Chapter 9 Fluorescent immunodetection of epigenetic modifications on preimplantation mouse embryos.
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    Chapter 10 Visualization of epigenetic modifications in preimplantation embryos.
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    Chapter 11 Live Embryo Imaging to Follow Cell Cycle and Chromosomes Stability After Nuclear Transfer
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    Chapter 12 Analysis of nucleolar morphology and protein localization as an indicator of nuclear reprogramming.
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    Chapter 13 Assessment of Cell Lineages and Cell Death in Blastocysts by Immunostaining
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    Chapter 14 Gene Expression Analysis in Early Embryos Through Reverse Transcription Quantitative PCR (RT-qPCR)
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    Chapter 15 Studying Bovine Early Embryo Transcriptome by Microarray
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    Chapter 16 Methylation of Specific Regions: Bisulfite-Sequencing at the Single Oocyte or 2-Cell Embryo Level
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    Chapter 17 Micro Chromatin Immunoprecipitation (μChIP) from Early Mammalian Embryos.
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    Chapter 18 Assessing reprogramming by chimera formation and tetraploid complementation.
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    Chapter 19 Whole-Mount In Situ Hybridization to Assess Advancement of Development and Embryo Morphology
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    Chapter 20 Genome-Wide Analysis of Methylation in Bovine Clones by Methylated DNA Immunoprecipitation (MeDIP)
Attention for Chapter 17: Micro Chromatin Immunoprecipitation (μChIP) from Early Mammalian Embryos.
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Chapter title
Micro Chromatin Immunoprecipitation (μChIP) from Early Mammalian Embryos.
Chapter number 17
Book title
Nuclear Reprogramming
Published in
Methods in molecular biology, October 2014
DOI 10.1007/978-1-4939-1594-1_17
Pubmed ID
25287350
Book ISBNs
978-1-4939-1593-4, 978-1-4939-1594-1
Authors

Dahl JA, Klungland A, John Arne Dahl, Arne Klungland, Dahl, John Arne, Klungland, Arne

Abstract

Chromatin immunoprecipitation (ChIP) is a powerful method for mapping protein-DNA interactions in vivo. Genomic localization of histone modifications, transcription factors, and other regulatory proteins can be revealed by ChIP. However, conventional ChIP protocols require the use of large numbers of cells, which prevents the application of ChIP to rare cell types. We have developed ChIP assays suited for the immunoprecipitation of histone proteins or transcription factors from small cell numbers. Here we describe a rapid, yet sensitive micro (μ)ChIP protocol producing high signal to noise ratio output, suitable for as few as 100 cells. This chapter provides a detailed protocol for μChIP from early mammalian embryos, also suitable for any sample of limited numbers of cells. Minor modifications of this optimized high signal to noise ChIP protocol make it a reliable tool for the use with any cell number (100-10(7)).

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 14 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 14 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 36%
Student > Master 3 21%
Researcher 3 21%
Professor 1 7%
Student > Bachelor 1 7%
Other 1 7%
Readers by discipline Count As %
Agricultural and Biological Sciences 7 50%
Biochemistry, Genetics and Molecular Biology 5 36%
Neuroscience 1 7%
Medicine and Dentistry 1 7%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 October 2014.
All research outputs
#20,239,689
of 22,766,595 outputs
Outputs from Methods in molecular biology
#9,865
of 13,090 outputs
Outputs of similar age
#213,119
of 255,128 outputs
Outputs of similar age from Methods in molecular biology
#95
of 130 outputs
Altmetric has tracked 22,766,595 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,090 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 255,128 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 130 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

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