Gene Correction
Humana Press
Chapter title |
Designing and Testing the Activities of TAL Effector Nucleases.
|
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Chapter number | 13 |
Book title |
Gene Correction
|
Published in |
Methods in molecular biology, February 2014
|
DOI | 10.1007/978-1-62703-761-7_13 |
Pubmed ID | |
Book ISBNs |
978-1-62703-760-0, 978-1-62703-761-7
|
Authors |
Lin Y, Cradick TJ, Bao G, Yanni Lin, Thomas J. Cradick, Gang Bao, Lin, Yanni, Cradick, Thomas J., Bao, Gang |
Abstract |
Transcription activator-like effector nucleases (TALENs) have rapidly developed into a powerful tool for genome editing. To avoid labor-intensive and time-consuming experimental screening for active TALENs, a scoring system can help select optimal target sites. Here we describe a procedure to design active TALENs using a scoring system named Scoring Algorithm for Predicted TALEN Activity (SAPTA) and a method to test the activity of individual and pairs of TALENs. |
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Portugal | 1 | 9% |
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Professor | 1 | 9% |
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