| Chapter title |
Analysis of Nucleocytoplasmic Protein Shuttling by Imaging Flow Cytometry
|
|---|---|
| Chapter number | 8 |
| Book title |
Imaging Flow Cytometry
|
| Published in |
Methods in molecular biology, January 2016
|
| DOI | 10.1007/978-1-4939-3302-0_8 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-3300-6, 978-1-4939-3302-0
|
| Authors |
Elizaveta Fasler-Kan, Yeldar Baiken, Ivan A. Vorobjev, Natasha S. Barteneva |
| Abstract |
Many intracellular signal transduction events involve the reversible shuttling of proteins between the cytoplasm and the nucleus. Study of these processes requires imaging information on the protein localization in a given cell and a large number of measurements to obtain sufficient statistics on the protein localization in the whole population. The protocol describes method for quantitative imaging flow cytometry analysis of intracellular distribution of NF-kappaB in ARPE-19 cells stained with specific fluorochrome-conjugated antibodies. The described technique alone or in combination with standard flow cytometry methods can be applied to study any protein undergoing translocation from cytoplasm into the nucleus in a variety of cell lines as well as in heterogeneous primary cell populations. |
Mendeley readers
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 5 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 1 | 20% |
| Student > Bachelor | 1 | 20% |
| Researcher | 1 | 20% |
| Student > Doctoral Student | 1 | 20% |
| Unknown | 1 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 2 | 40% |
| Biochemistry, Genetics and Molecular Biology | 1 | 20% |
| Physics and Astronomy | 1 | 20% |
| Unknown | 1 | 20% |