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X Demographics
Mendeley readers
Attention Score in Context
| Chapter title |
Identification of Novel Protein Functions and Signaling Mechanisms by Genetics and Quantitative Phosphoproteomics in Caenorhabditis elegans
|
|---|---|
| Chapter number | 9 |
| Book title |
Stable Isotope Labeling by Amino Acids in Cell Culture (SILAC)
|
| Published in |
Methods in molecular biology, January 2014
|
| DOI | 10.1007/978-1-4939-1142-4_9 |
| Pubmed ID | |
| Book ISBNs |
978-1-4939-1141-7, 978-1-4939-1142-4
|
| Authors |
Julius Fredens, Kasper Engholm-Keller, Jakob Møller-Jensen, Martin Røssel Larsen, Nils J. Færgeman |
| Abstract |
Stable isotope labeling by amino acids combined with mass spectrometry is a widely used methodology for measuring relative changes in protein and phosphorylation levels at a global level. We have applied this method to the model organism Caenorhabditis elegans in combination with RNAi-mediated gene knockdown by feeding the nematode on pre-labeled lysine auxotroph Escherichia coli. In this chapter, we describe in details the generation of the E. coli strain, incorporation of heavy isotope-labeled lysine in C. elegans, and the procedure for a comprehensive global phosphoproteomic experiment. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 15 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 3 | 20% |
| Researcher | 3 | 20% |
| Student > Ph. D. Student | 2 | 13% |
| Other | 1 | 7% |
| Student > Doctoral Student | 1 | 7% |
| Other | 2 | 13% |
| Unknown | 3 | 20% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 5 | 33% |
| Agricultural and Biological Sciences | 3 | 20% |
| Veterinary Science and Veterinary Medicine | 1 | 7% |
| Immunology and Microbiology | 1 | 7% |
| Medicine and Dentistry | 1 | 7% |
| Other | 2 | 13% |
| Unknown | 2 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 September 2014.
All research outputs
#15,304,580
of 22,761,738 outputs
Outputs from Methods in molecular biology
#5,318
of 13,088 outputs
Outputs of similar age
#190,042
of 305,296 outputs
Outputs of similar age from Methods in molecular biology
#200
of 596 outputs
Altmetric has tracked 22,761,738 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,088 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 305,296 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 596 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 56% of its contemporaries.