Chapter title |
Fluorescence activated cell sorting of shoot apical meristem cell types.
|
---|---|
Chapter number | 17 |
Book title |
Flower Development
|
Published in |
Methods in molecular biology, January 2014
|
DOI | 10.1007/978-1-4614-9408-9_17 |
Pubmed ID | |
Book ISBNs |
978-1-4614-9407-2, 978-1-4614-9408-9
|
Authors |
Reddy GV, G. Venugopala Reddy |
Editors |
José Luis Riechmann, Frank Wellmer |
Abstract |
Growing tips of plants harbor a set of stem cells in structures called shoot apical meristems (SAMs) which provide cells for development of aboveground biomass. Despite a periodic differentiation of stem cell progenitors into leaves, the stem cell pool remains constant over time. Genetic analysis has revealed molecular pathways involved in stem-cell specification, cell division patterns, and organ differentiation. Stem cells within SAMs are few in number, which imposes a limitation to the experimental approaches that can be used for deciphering the gene regulatory networks that underlie cell fate transitions. Here, I provide detailed experimental protocols for the protoplasting and subsequent purification through cell sorting of SAM cells, which allows genome-wide analyses of gene expression patterns at a single cell-type resolution. |
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