| Chapter title |
Next-Generation Sequencing Applied to Flower Development: RNA-Seq.
|
|---|---|
| Chapter number | 23 |
| Book title |
Flower Development
|
| Published in |
Methods in molecular biology, January 2014
|
| DOI | 10.1007/978-1-4614-9408-9_23 |
| Pubmed ID | |
| Book ISBNs |
978-1-4614-9407-2, 978-1-4614-9408-9
|
| Authors |
Jun He, Yuling Jiao, He, Jun, Jiao, Yuling |
| Editors |
José Luis Riechmann, Frank Wellmer |
| Abstract |
Genome-wide study of gene expression, or transcriptome profiling, is critical for our understanding of biological functions, including developmental processes. Recent breakthroughs in high-throughput sequencing technologies have revolutionized gene expression profiling to study the transcriptome at the nucleotide level, which is known as RNA-seq. RNA-seq, also called "whole transcriptome shotgun sequencing," uses next-generation sequencing technologies to sequence cDNA in order to infer a sample's RNA content. Here we describe a detailed bench-ready protocol to generate RNA-seq libraries for high-throughput single-end or pair-end sequencing compatible with the Illumina sequencing platform. |
X Demographics
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|---|---|---|
| India | 1 | 100% |
Demographic breakdown
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|---|---|---|
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Mendeley readers
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|---|---|---|
| Japan | 1 | 7% |
| Unknown | 13 | 93% |
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|---|---|---|
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| Lecturer | 1 | 7% |
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| Professor | 1 | 7% |
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| Unknown | 3 | 21% |
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| Unknown | 4 | 29% |