Chapter title |
Measurement of Tricarboxylic Acid Cycle Enzyme Activities in Plants
|
---|---|
Chapter number | 14 |
Book title |
Plant Respiration and Internal Oxygen
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7292-0_14 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7291-3, 978-1-4939-7292-0
|
Authors |
Rebeca Patricia Omena-Garcia, Wagner L. Araújo, Yves Gibon, Alisdair R. Fernie, Adriano Nunes-Nesi |
Abstract |
Mitochondria are vital cytoplasmic organelle of eukaryotic cells responsible for oxidative energy metabolism and the synthesis of intermediates utilized in various other metabolic pathways. The functions of mitochondrion are the oxidation of organic acids by the tricarboxylic acid (TCA) cycle and the synthesis of ATP by the oxidative phosphorylation in the mitochondrial electron transport chain. The TCA cycle is composed by a set of enzymes that are essential for optimal functioning of the primary carbon metabolism in plants. The activity of each TCA cycle enzyme in plants may vary according to cell type, plant tissue, stage of plant development, and the environment. Here, we describe current methods used for the determination of the TCA cycle enzyme activities in different plant tissues. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 20 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 4 | 20% |
Student > Ph. D. Student | 3 | 15% |
Other | 2 | 10% |
Professor | 2 | 10% |
Student > Doctoral Student | 1 | 5% |
Other | 3 | 15% |
Unknown | 5 | 25% |
Readers by discipline | Count | As % |
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Business, Management and Accounting | 1 | 5% |
Psychology | 1 | 5% |
Other | 1 | 5% |
Unknown | 6 | 30% |