Chapter title |
High-Resolution Chromatin Immunoprecipitation: ChIP-Sequencing
|
---|---|
Chapter number | 6 |
Book title |
Methods in Molecular Biology
|
Published in |
Methods in molecular biology, August 2017
|
DOI | 10.1007/978-1-4939-7098-8_6 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7097-1, 978-1-4939-7098-8
|
Authors |
Diaz, Roxanne E., Sanchez, Aurore, Anton Le Berre, Véronique, Bouet, Jean-Yves, Roxanne E. Diaz, Aurore Sanchez, Véronique Anton Le Berre, Jean-Yves Bouet |
Abstract |
Chromatin immunoprecipitation (ChIP) coupled with next-generation sequencing (NGS) is widely used for studying the nucleoprotein components that are involved in the various cellular processes required for shaping the bacterial nucleoid. This methodology, termed ChIP-sequencing (ChIP-seq), enables the identification of the DNA targets of DNA binding proteins across genome-wide maps. Here, we describe the steps necessary to obtain short, specific, high-quality immunoprecipitated DNA prior to DNA library construction for NGS and high-resolution ChIP-seq data. |
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