Chapter title |
Production of Minicircle DNA Vectors Using Site-Specific Recombinases
|
---|---|
Chapter number | 20 |
Book title |
Site-Specific Recombinases
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7169-5_20 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7167-1, 978-1-4939-7169-5
|
Authors |
Nafiseh Sanei Ata-abadi, Naeimeh Rezaei, Kianoush Dormiani, Mohammad Hossein Nasr-Esfahani, Ata-abadi, Nafiseh Sanei, Rezaei, Naeimeh, Dormiani, Kianoush, Nasr-Esfahani, Mohammad Hossein |
Abstract |
Minicircle DNA vectors are plasmid derivatives free of bacterial elements. These vectors are mostly provided from common plasmids via recombination by site-specific recombinases in E. coli. Absence of bacterial backbone in minicircle vectors results in high-level and persistent expression of transgene in comparison with conventional plasmids and provides promising vehicles for gene therapy and vaccination. Here we describe the production of replicative minicircle DNA vectors using the PBAD/araC system expressing ΦC31 integrase in E. coli. |
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