Chapter title |
Preparing Mate-Paired Illumina Libraries Using Cre Recombinase
|
---|---|
Chapter number | 16 |
Book title |
Site-Specific Recombinases
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7169-5_16 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7167-1, 978-1-4939-7169-5
|
Authors |
Ze Peng, Nandita Nath, Zhiying Zhao, Jeff L. Froula, Jan-Fang Cheng, Feng Chen |
Abstract |
Large insert mate pair reads have been used in de novo assembly and discovery of structural variants. We developed a new approach, Cre-LoxP inverse PCR paired end (CLIP-PE), which exploits the advantages of (1) Cre-LoxP recombination system to efficiently circularize large DNA fragments, (2) inverse PCR to enrich for the desired products that contain both ends of the large DNA fragments, and (3) use of restriction enzymes to introduce a recognizable junction site between ligated fragment ends. We have successfully created CLIP-PE libraries of up to 22 kb jumping pairs and demonstrated their ability to improve genome assemblies. The CLIP-PE methodology can be implemented with existing and future next-generation sequencing platforms. |
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