Chapter title |
Recombinase-Mediated Cassette Exchange Using Adenoviral Vectors
|
---|---|
Chapter number | 9 |
Book title |
Site-Specific Recombinases
|
Published in |
Methods in molecular biology, January 2017
|
DOI | 10.1007/978-1-4939-7169-5_9 |
Pubmed ID | |
Book ISBNs |
978-1-4939-7167-1, 978-1-4939-7169-5
|
Authors |
Andreas F. Kolb, Christopher Knowles, Patrikas Pultinevicius, Jennifer A. Harbottle, Linda Petrie, Claire Robinson, David A. Sorrell, Kolb, Andreas F., Knowles, Christopher, Pultinevicius, Patrikas, Harbottle, Jennifer A., Petrie, Linda, Robinson, Claire, Sorrell, David A. |
Abstract |
Site-specific recombinases are important tools for the modification of mammalian genomes. In conjunction with viral vectors, they can be utilized to mediate site-specific gene insertions in animals and in cell lines which are difficult to transfect. Here we describe a method for the generation and analysis of an adenovirus vector supporting a recombinase-mediated cassette exchange reaction and discuss the advantages and limitations of this approach. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 5 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 1 | 20% |
Lecturer | 1 | 20% |
Other | 1 | 20% |
Student > Master | 1 | 20% |
Unknown | 1 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 40% |
Agricultural and Biological Sciences | 2 | 40% |
Unknown | 1 | 20% |