↓ Skip to main content
What is this page? Embed badge

Site-Specific Recombinases

Overview of attention for book
Cover of 'Site-Specific Recombinases'

Table of Contents

  1. Altmetric Badge
    Book Overview
  2. Altmetric Badge
    Chapter 1 Generating Genetically Modified Mice: A Decision Guide
  3. Altmetric Badge
    Chapter 2 Direct Generation of Conditional Alleles Using CRISPR/Cas9 in Mouse Zygotes
  4. Altmetric Badge
    Chapter 3 Building Cre Knockin Rat Lines Using CRISPR/Cas9
  5. Altmetric Badge
    Chapter 4 Dual Recombinase-Mediated Cassette Exchange by Tyrosine Site-Specific Recombinases
  6. Altmetric Badge
    Chapter 5 Use of the DICE (Dual Integrase Cassette Exchange) System
  7. Altmetric Badge
    Chapter 6 Ligand-Controlled Site-Specific Recombination in Zebrafish
  8. Altmetric Badge
    Chapter 7 Injection-Based Delivery of Cell-Permeable Peptide-Tagged Cre
  9. Altmetric Badge
    Chapter 8 Viral Delivery of GFP-Dependent Recombinases to the Mouse Brain
  10. Altmetric Badge
    Chapter 9 Recombinase-Mediated Cassette Exchange Using Adenoviral Vectors
  11. Altmetric Badge
    Chapter 10 Marker Removal in Transgenic Plants Using Cre Recombinase Delivered with Potato Virus X
  12. Altmetric Badge
    Chapter 11 Nanoparticle-Mediated Recombinase Delivery into Maize
  13. Altmetric Badge
    Chapter 12 Immunohistochemical Procedures for Characterizing the Retinal Expression Patterns of Cre Driver Mouse Lines
  14. Altmetric Badge
    Chapter 13 FLPing Genes On and Off in Drosophila
  15. Altmetric Badge
    Chapter 14 Imaging Neural Architecture in Brainbow Samples
  16. Altmetric Badge
    Chapter 15 Purification and In Vitro Characterization of Zinc Finger Recombinases
  17. Altmetric Badge
    Chapter 16 Preparing Mate-Paired Illumina Libraries Using Cre Recombinase
  18. Altmetric Badge
    Chapter 17 Preparing Fosmid Mate-Paired Libraries Using Cre-LoxP Recombination
  19. Altmetric Badge
    Chapter 18 Using Purified Tyrosine Site-Specific Recombinases In Vitro to Rapidly Construct and Diversify Metabolic Pathways
  20. Altmetric Badge
    Chapter 19 Multipart DNA Assembly Using Site-Specific Recombinases from the Large Serine Integrase Family
  21. Altmetric Badge
    Chapter 20 Production of Minicircle DNA Vectors Using Site-Specific Recombinases
Attention for Chapter 10: Marker Removal in Transgenic Plants Using Cre Recombinase Delivered with Potato Virus X
Altmetric Badge

Mentioned by

twitter
1 X user

Citations

dimensions_citation
1 Dimensions

Readers on

mendeley
6 Mendeley
Summary X Dimensions citations
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Chapter title
Marker Removal in Transgenic Plants Using Cre Recombinase Delivered with Potato Virus X
Chapter number 10
Book title
Site-Specific Recombinases
Published in
Methods in molecular biology, January 2017
DOI 10.1007/978-1-4939-7169-5_10
Pubmed ID
28815499
Book ISBNs
978-1-4939-7167-1, 978-1-4939-7169-5
Authors

Lilya Kopertekh, Joachim Schiemann

Abstract

In this chapter we present an alternative method to develop marker-free transgenic plants. It makes use of the Cre/loxP recombination system from bacteriophage P1 and consists of two essential components. The first component is the transgenic plant containing a loxP-flanked marker gene. The second component is a cre transient expression vector based on potato virus X. The great benefit of this transient delivery method consists in the avoidance of stable integration of the cre recombinase gene into the plant genome. Upon infection of the loxP-target plant with PVX-Cre, the virus spreads systemically through the plant and causes the recombinase-mediated excision of the marker gene. Marker-free transgenic loci can be transmitted to the progeny by plant regeneration from PVX-Cre systemically infected leaves or self-pollination of virus-infected plants. The protocol covers generation of loxP-target transgenic plants, PVX-mediated delivery of Cre recombinase protein, phenotypic and molecular analysis of recombination events, and transmission of marker-free transgenic loci to the next generation. The transient expression system described in this chapter can be adapted for marker gene removal in other plant species that are amenable for virus infection.

View on publisher site
X Demographics

X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
 Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 6 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 6 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 17%
Professor > Associate Professor 1 17%
Researcher 1 17%
Student > Doctoral Student 1 17%
Unknown 2 33%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 50%
Unknown 3 50%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 August 2017.
All research outputs
#20,442,790
of 22,997,544 outputs
Outputs from Methods in molecular biology
#9,933
of 13,151 outputs
Outputs of similar age
#356,128
of 421,196 outputs
Outputs of similar age from Methods in molecular biology
#842
of 1,074 outputs
Altmetric has tracked 22,997,544 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,151 research outputs from this source. They receive a mean Attention Score of 3.4. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 421,196 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,074 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.

This page is provided by Altmetric.