Title |
A Highly Sensitive Electrochemical DNA Biosensor from Acrylic-Gold Nano-composite for the Determination of Arowana Fish Gender
|
---|---|
Published in |
Discover Nano, August 2017
|
DOI | 10.1186/s11671-017-2254-y |
Pubmed ID | |
Authors |
Mahbubur Rahman, Lee Yook Heng, Dedi Futra, Chew Poh Chiang, Zulkafli A. Rashid, Tan Ling Ling |
Abstract |
The present research describes a simple method for the identification of the gender of arowana fish (Scleropages formosus). The DNA biosensor was able to detect specific DNA sequence at extremely low level down to atto M regimes. An electrochemical DNA biosensor based on acrylic microsphere-gold nanoparticle (AcMP-AuNP) hybrid composite was fabricated. Hydrophobic poly(n-butylacrylate-N-acryloxysuccinimide) microspheres were synthesised with a facile and well-established one-step photopolymerization procedure and physically adsorbed on the AuNPs at the surface of a carbon screen printed electrode (SPE). The DNA biosensor was constructed simply by grafting an aminated DNA probe on the succinimide functionalised AcMPs via a strong covalent attachment. DNA hybridisation response was determined by differential pulse voltammetry (DPV) technique using anthraquinone monosulphonic acid redox probe as an electroactive oligonucleotide label (Table 1). A low detection limit at 1.0 × 10(-18) M with a wide linear calibration range of 1.0 × 10(-18) to 1.0 × 10(-8) M (R (2) = 0.99) can be achieved by the proposed DNA biosensor under optimal conditions. Electrochemical detection of arowana DNA can be completed within 1 hour. Due to its small size and light weight, the developed DNA biosensor holds high promise for the development of functional kit for fish culture usage. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 33 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 6 | 18% |
Other | 4 | 12% |
Student > Doctoral Student | 4 | 12% |
Student > Master | 3 | 9% |
Student > Bachelor | 2 | 6% |
Other | 4 | 12% |
Unknown | 10 | 30% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 4 | 12% |
Agricultural and Biological Sciences | 4 | 12% |
Engineering | 3 | 9% |
Biochemistry, Genetics and Molecular Biology | 2 | 6% |
Unspecified | 2 | 6% |
Other | 4 | 12% |
Unknown | 14 | 42% |