Advances in experimental medicine and biology

Chapter 1 The Complex Between Mesentericopeptidase and Eglin-C

Chapter 2 Crystallographic Study of Eglin-C Binding to Thermitase

Chapter 3 Design of Specific Peptide Structures and Subtilisin Enzyme Inhibitors Using α, β-Dehydro-Residues

Chapter 4 Structure and Function of Subtilisin BPN’ as Studied Through Crystallographic Studies on a Series of its Complexes with Genetically Engineered Proteinaceous Inhibitor SSI

Chapter 5 Three Dimensional Structure of the Antibiotic Bacitracin a Complexed to Two Different Subtilisin Proteases: Novel Mode of Enzyme Inhibition

Chapter 6 Active Site Binding Loop Stabilization in the Subtilisin Inhibitor Eglin C: Structural and Functional Studies on Specifically Designed Mutants in Complex with Subtilisin and the Uncomplexed Inhibitor

Chapter 7 Crystal Structures of the Alkaline Proteases Savinase and Esperase from Bacillus Lentus

Chapter 8 Modelling and Engineering of Enzyme/Substrate Interactions in Subtilisin-Like Enzymes of Unknown 3-Dimensional Structure

Chapter 9 Subtilases: Subtilisin-Like Serine Proteases

Chapter 10 Cloning and Expression of the Gene Encoding a Novel Proteinase from TritiraChium Album Limber

Chapter 11 Studies of Binding Sites in the Subtilisin from Bacillus Lentus by Means of Site Directed Mutagenesis and Kinetic Investigations

Chapter 12 Laundry Performance Of Subtilisin Proteases

Chapter 13 Free Energy Perturbation Techniques Applied To Subtilisin BPN’ Stability

Chapter 14 Thermitase — Kinetic Differentiation to the Subtilisins

Chapter 15 Fluorescence Decay of Tryptophans in Serine Proteinases from Microorganisms: Relation to X-Ray Models

Chapter 16 Propeptide-Mediated Folding in Subtilisin: The Intramolecular Chaperone Concept

Chapter 17 Roles of The N- and C- Terminal Pro-Sequences of Aqualysin I Precursor in the Processing and Extracellular Secretion of the Enzyme

Chapter 18 Crystal Structure Analysis of Subtilisin BPN’ Mutants Engineered for Studying Thermal Stability

Chapter 19 Random Mutagenesis of the Weak Calcium Binding Site in Subtilisin Carlsberg and Screening for Thermostability by Temperature-Gradient Gel Electrophoresis

Chapter 20 Inhibition of Proteinase K by Mercury

Chapter 21 Crystallographic Investigations of Subtilisin BPN’ Mutants Engineered for Studying Thermal Stability

Chapter 22 Structural and Functional Consequences of Engineering the High Alkaline Serine Protease PB92

Chapter 23 Engineering Surface Charges in a Subtilisin

Chapter 24 PH Dependence of the Catalytic Activity of a Subtilisin-Like Proteinase

Chapter 25 An Investigation of the Savinase Water Channel: Implications of Cavity Mutations

Chapter 26 Substrate Specificity of Natural Variants and Genetically Engineered Intermediates of Bacillus Lentus Alkaline Proteases

Chapter 27 Unusual Ligand Binding at the Active Site Domain of an Engineered Mutant of Subtilisin BL

Chapter 28 Studies on the Structure and Function of Subtilisin E by Protein Engineering

Chapter 29 Structural Changes Leading to Increased Enzymatic Activity in an Engineered Variant of Bacillus Lentus Subtilisin