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RNA Remodeling Proteins

Overview of attention for book
Cover of 'RNA Remodeling Proteins'

Table of Contents

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    Book Overview
  2. Altmetric Badge
    Chapter 1 Key Points to Consider When Studying RNA Remodeling by Proteins
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    Chapter 2 Happy Birthday: 25 Years of DEAD-Box Proteins.
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    Chapter 3 In Vivo Cross-Linking Followed by PolyA Enrichment to Identify Yeast mRNA Binding Proteins.
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    Chapter 4 Dynamics of the spb4 interactome monitored by affinity purification.
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    Chapter 5 Using EMOTE to Map the Exact 5′-Ends of Processed RNA on a Transcriptome-Wide Scale
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    Chapter 6 Cellular Localization of RNA Degradation and Processing Components in Escherichia coli
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    Chapter 7 Recombineering Applications for the Mutational Analysis of Bacterial RNA-Binding Proteins and Their Sites of Action
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    Chapter 8 Determination of RNA Chaperone Activity Using an Escherichia coli Mutant
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    Chapter 9 Biochemical Characterization of G4 Quadruplex Telomerase RNA Unwinding by the RNA Helicase RHAU
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    Chapter 10 ATPase Site Configuration of the RNA Helicase DbpA Probed by ENDOR Spectroscopy
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    Chapter 11 Bioinformatics and Biochemical Methods to Study the Structural and Functional Elements of DEAD-Box RNA Helicases
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    Chapter 12 Measuring Helicase Inhibition of the DEAD-Box Protein Dbp2 by Yra1.
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    Chapter 13 A FRET-Based, Continuous Assay for the Helicase Activity of DEAD-Box Proteins
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    Chapter 14 A Fluorescence-Based Screening Assay for Identification of Hepatitis C Virus NS3 Helicase Inhibitors and Characterization of Their Inhibitory Mechanism
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    Chapter 15 Mechanisms of HCV NS3 Helicase Monitored by Optical Tweezers
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    Chapter 16 Constructing a Magnetic Tweezers to Monitor RNA Translocation at the Single-Molecule Level
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    Chapter 17 Probing RNA Translocases with DNA
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    Chapter 18 Monitoring RNA Unwinding by the Transcription Termination Factor Rho from Mycobacterium tuberculosis
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    Chapter 19 Characterization of the mechanisms of transcription termination by the helicase sen1.
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    Chapter 20 Characterization of TRAP-Mediated Regulation of the B. subtilis trp Operon Using In Vitro Transcription and Transcriptional Reporter Fusions In Vivo
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    Chapter 21 Single-Molecule FRET Characterization of RNA Remodeling Induced by an Antitermination Protein
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    Chapter 22 Fluorescence Reporters for Hfq Oligomerization and RNA Annealing
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    Chapter 23 Fluorescence anisotropy-based salt-titration approach to characterize protein-nucleic Acid interactions.
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    Chapter 24 Probing Hfq:RNA Interactions with Hydroxyl Radical and RNase Footprinting.
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    Chapter 25 Purification of Eukaryotic Exoribonucleases Following Heterologous Expression in Bacteria and Analysis of Their Biochemical Properties by In Vitro Enzymatic Assays
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    Chapter 26 Tips and Tricks to Probe the RNA-Degrading Activities of Hyperthermophilic Archaeal β-CASP Ribonucleases.
Attention for Chapter 3: In Vivo Cross-Linking Followed by PolyA Enrichment to Identify Yeast mRNA Binding Proteins.
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Chapter title
In Vivo Cross-Linking Followed by PolyA Enrichment to Identify Yeast mRNA Binding Proteins.
Chapter number 3
Book title
RNA Remodeling Proteins
Published in
Methods in molecular biology, January 2015
DOI 10.1007/978-1-4939-2214-7_3
Pubmed ID
Book ISBNs
978-1-4939-2213-0, 978-1-4939-2214-7
Authors

Mitchell SF, Parker R, Sarah F. Mitchell, Roy Parker

Abstract

mRNA binding proteins regulate gene expression by controlling the processing, localization, decay, and translation of messenger RNAs (mRNAs). To fully understand this process, it is necessary to identify the complete set of mRNA binding proteins. This work describes a method for the systematic identification of yeast mRNA binding proteins. This method applies in vivo UV cross-linking, affinity pull-down of polyA(+) mRNAs, and analysis by mass spectrometry to identify proteins that directly bind to mRNAs.

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X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 3 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 1 33%
Professor > Associate Professor 1 33%
Researcher 1 33%
Readers by discipline Count As %
Agricultural and Biological Sciences 2 67%
Biochemistry, Genetics and Molecular Biology 1 33%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 13 January 2015.
All research outputs
#17,737,508
of 22,778,347 outputs
Outputs from Methods in molecular biology
#7,192
of 13,093 outputs
Outputs of similar age
#242,235
of 353,085 outputs
Outputs of similar age from Methods in molecular biology
#431
of 1,008 outputs
Altmetric has tracked 22,778,347 research outputs across all sources so far. This one is in the 19th percentile – i.e., 19% of other outputs scored the same or lower than it.
So far Altmetric has tracked 13,093 research outputs from this source. They receive a mean Attention Score of 3.3. This one is in the 39th percentile – i.e., 39% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 353,085 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 1,008 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.