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Chapter title |
Genome manipulations with bacterial recombineering and site-specific integration in Drosophila.
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Chapter number | 2 |
Book title |
Gene Correction
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Published in |
Methods in molecular biology, February 2014
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DOI | 10.1007/978-1-62703-761-7_2 |
Pubmed ID | |
Book ISBNs |
978-1-62703-760-0, 978-1-62703-761-7
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Authors |
Zhang Y, Schreiner W, Rong YS, Yi Zhang, William Schreiner, Yikang S. Rong, Zhang, Yi, Schreiner, William, Rong, Yikang S. |
Abstract |
Gene targeting is a vital tool for modern biology. The ability to efficiently and repeatedly target the same locus is made more efficient by the site-specific integrase mediated repeated targeting (SIRT) method, which combines homologous recombination, site-specific integration, and bacterial recombineering to conduct targeted modifications of individual loci. Here we describe the recombineering designs and procedures for the introduction of epitope tags, in-frame deletion mutations, and point mutations into plasmids that can later be used for SIRT. |
Mendeley readers
The data shown below were compiled from readership statistics for 3 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
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Unknown | 3 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 2 | 67% |
Researcher | 1 | 33% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 3 | 100% |